Numerical Modeling and Spectral Balancing of an LED-Based Reflectance Spectrophotometer (360–760 nm)

Authors

DOI:

https://doi.org/10.18287/JBPE26.12.020311

Keywords:

reflectance spectrophotometry, diffuse reflection, optical design, CCD detector, constrained optimization

Abstract

This study presents numerical modeling and constrained spectral balancing of a compact LED-based reflectance spectrophotometer operating in the 360–760 nm range. The detected spectral response was modeled as a weighted superposition of individual LED emission spectra explicitly multiplied by the wavelength-dependent detector quantum efficiency QE(λ). In the baseline configuration with unit weights, the detected spectrum exhibited strong modulation, characterized by a coefficient of variation CV of 41.5%, peak-to-peak deviation of 131%, and maximum-to-minimum ratio of 4.24. Spectral balancing was formulated as a constrained minimization of the normalized variance over a 0.5 nm wavelength grid using a differential evolution algorithm with bounded non-negative weights. The optimized configuration reduced CV to 8.36%, peak-to-peak deviation to 32.2%, and max/min ratio to 1.39, corresponding to an approximately 80% reduction in global spectral non-uniformity as quantified by variance-based metrics. The proposed approach provides a quantitative methodology for spectral equalization in multi-LED reflectance spectrophotometers.

Author Biographies

  • Mikhail E. Parfyonov, Samara National Research University, Russian Federation
    PhD student, department of Laser and Biotechnical Systems
  • Dmitry N. Artemyev, Samara National Research University, Russian Federation
    PhD, department of Laser and Biotechnical Systems

References

T. A. Germer, J. C. Zwinkels, and B. K. Tsai (Eds.), Spectrophotometry: Accurate Measurement of Optical Properties of Materials, vol. 46, Elsevier, Oxford, UK (2014). ISBN: 978-0-12-386022-4.

G. Kortüm, Reflectance Spectroscopy: Principles, Methods, Applications, Springer, Berlin, Heidelberg (2012). ISBN: 978-3-642-88073-5.

A. Springsteen, Applied Spectroscopy: A Compact Reference for Practitioners, Academic Press, 193–224 (1998). ISBN: 978-0-12-764070-9.

I. Badura, M. Dąbski, “Reflectance spectroscopy in geology and soil sciences: literature review,” Quaestiones Geographicae 41(3), 157–167 (2022).

M. Witteveen, D. J. Faber, H. J. C. M. Sterenborg, T. J. M. Ruers, T. G. Van Leeuwen, and A. L. Post, “Opportunities and pitfalls in (sub)diffuse reflectance spectroscopy,” Frontiers in Photonics 3, 964719 (2022).

E.W. Ciurczak, B. Igne, J. Workman, and D.A. Burns (Eds.), Handbook of Near-Infrared Analysis, 4th ed., CRC Press, Boca Raton (2021). ISBN: 978-1-35-126988-9.

M. B. Wallace, A. Wax, D. N. Roberts, and R. N. Graf, “Reflectance spectroscopy,” Gastrointestinal Endoscopy Clinics of North America 19(2), 233–242 (2009).

I. T. Young, Y. Garini, H. R. C. Dietrich, W. Van Oel, and G. Liqui Lung, LEDs for fluorescence microscopy, Proceedings of SPIE, 5324, 208 (2004).

W. Demtröder, Laser Spectroscopy, Springer, Berlin, Heidelberg (2003). ISBN: 978-3-540-73418-5

E. F. Schubert, Light-Emitting Diodes, 2nd ed., Cambridge University Press (2006). ISBN: 978-0-511-79054-6

S. Pimputkar, J. S. Speck, S. P. DenBaars, and S. Nakamura, “Prospects for LED lighting,” Nature Photonics 3, 180–182 (2009).

Y. Ryu, D. D. Baldocchi, J. Verfaillie, S. Ma, M. Falk, I. Ruiz-Mercado, T. Hehn, and O. Sonnentag, “Testing the performance of a novel spectral reflectance sensor, built with light emitting diodes (LEDs), to monitor ecosystem metabolism, structure and function,” Agricultural and Forest Meteorology 150(12), 1597–1606 (2010).

S. D. Noble, R. B. Brown, and T. G. Crowe, “Design and evaluation of an imaging spectrophotometer incorporating a uniform light source,” Review of Scientific Instruments 83(3), 033112 (2012).

M. Naeem, T. Imran, M. Hussain, and A. S. Bhatti, “Design simulation and data analysis of an optical spectrometer,” Optics 3(3), 304–312 (2022).

M. Czerny, A. F. Turner, “Über den astigmatismus bei spiegelspektrometern,” Zeitschrift für Physik 61, 792–797 (1930).

P. R. Bevington, D. K. Robinson, Data Reduction and Error Analysis for the Physical Sciences, McGraw-Hill Education, (2003). ISBN: 978-0-07-247227-1.

R. Storn, K. Price, “Differential evolution – a simple and efficient heuristic for global optimization over continuous spaces,” Journal of Global Optimization 11(4), 341–359 (1997).

Downloads

Published

2026-07-02

Issue

Section

Special Section

How to Cite

Numerical Modeling and Spectral Balancing of an LED-Based Reflectance Spectrophotometer (360–760 nm). (2026). Journal of Biomedical Photonics & Engineering, 12(2), 020311. https://doi.org/10.18287/JBPE26.12.020311